AdministratorWhat is a TGX gel?
Description. Long shelf life Mini-PROTEAN® TGX™ Precast Gels are an innovative PAGE system designed to provide very fast separation while maintaining high resolution in standard Tris-glycine buffer. Mini-PROTEAN Precast Gels are compatible with Mini-PROTEAN Tetra (1–4 gels) and Mini-PROTEAN® Dodeca™ (1–12 gels) Cells.
What is TGX Stain-Free gel?
What Is Stain-Free Technology? Mini-PROTEAN TGX Stain-Free Precast Gels are based on the long-shelf-life TGX (Tris-Glycine eXtended) formulation and include unique trihalo compounds that allow rapid fluorescent detection of proteins with Bio-Rad stain-free imaging systems.
What is a criterion gel?
Criterion precast gels include a broad selection of mid-sized polyacrylamide gels in cassettes with integrated upper chambers. Individually packaged in various formulations, these gels can run up to 26 samples.
What is stain-free gel?
Stain-Free imaging technology utilizes a polyacrylamide gel containing a proprietary trihalo compound to make proteins fluorescent directly in the gel with a short photoactivation, allowing the immediate visualization of proteins at any point during electrophoresis and western blotting.
What is Coomassie blue staining?
Coomassie blue dyes are a family of dyes commonly used to stain proteins in SDS-PAGE gels. The gels are soaked in dye, and excess stain is then eluted with a solvent (“destaining”). This treatment allows the visualization of proteins as blue bands on a clear background.
How long is the run time for the mini gel?
4. Run gel: Prepare the samples in Laemmli sample buffer and load into the wells. Run the gel at 200–400 V constant voltage (see protocols on page 4) until the dye front reaches the reference line (approximately 10–30 min). At the completion of the run, disconnect the cell and remove the cassette.
How do you visualize stain free gel?
In order to visualize protein bands using stain-free technology on gels and blots (if applicable), stain-free gels must be activated after electrophoretic separation is complete. Once activated, the same protein bands can be visualized (without any further activation) on blotting membranes after transfer.
What is Coomassie used for?
Description. Coomassie blue dyes are a family of dyes commonly used to stain proteins in SDS-PAGE gels. The gels are soaked in dye, and excess stain is then eluted with a solvent (“destaining”). This treatment allows the visualization of proteins as blue bands on a clear background.