AdministratorHow does cordycepin inhibit DNA synthesis?
Cordycepin is an adenosine analogue, which is readily phosphorylated to its mono, di and triphosphate form intracellularly. Triphosphate Cordycepin can be incorporated into RNA and inhibits transcription elongation and RNA synthesis due to the absence of hydroxyl moiety at the 3′ position.
What does cordycepin inhibit?
A, protein synthesis rates of NIH3T3 cells as measured by 35S incorporation into protein, corrected for total protein concentration. The effects of treatment with 200 μm cordycepin or adenosine or with 10 μg/ml actinomycin D (AcD) are shown.
How do you extract cordycepin from Cordyceps?
Cordycepin was separated successfully from Cordyceps militaris using extraction with a mixture of ethanol and water. Optimum temperature, composition of ethanol in water, solvent/solid ratio and pH of solvent were determined by a central composite design (CCD) with respect to extraction yield.
Does 3 Deoxyadenosine inhibit DNA synthesis?
A structural analogue of ATP, 3′-deoxyadenosine triphosphate (3′-dATP), has been synthesized from cordycepin (3′-deoxyadenosine), characterized, and determined to be an inhibitor of ATP-dependent DNA synthesis in Escherichia coli cells which have been reduced permeable to nucleoside triphosphates by treatment with …
Is Cordycepin an antibiotic?
A purine nucleoside antimetabolite and antibiotic isolated from the fungus Cordyceps militaris with potential antineoplastic, antioxidant, and anti-inflammatory activities.
How do you increase Cordycepin level in Cordyceps Militaris?
Thus, researches to increase the natural cordycepin content in C. militaris have been peformed through various methods such as breeding by mating or genetic manipulation and C. militaris with higher production of cordycepin is very valuable in the health food or medicine industry [9,10].
Is Cordycepin a polysaccharide?
Cordyceps polysaccharides mainly include extracellular polysaccharide and intracellular polysaccharide.
What is difference between ATP and dATP?
The main difference between ATP and dATP is that ATP is a ribonucleotide whereas dATP is a deoxyribonucleotide. And, a functional difference between them is that ATP serves as the energy currency of the cell as well as a building block of RNA and dATP serves as a precursor for DNA synthesis.
Why is ATP not called dATP?
Definition. ATP refers to the phosphorylated nucleotide that composes adenosine and three phosphate groups and supplies energy for many biochemical, cellular process, especially to ADP, by undergoing enzymatic hydrolysis. In contrast, dATP refers to one of the two purine nucleotides that are used to synthesize DNA.
Is Cordycepin water soluble?
This product is soluble in water (1 mg/ml). The solubility is routinely tested at 20 mg/ml (1 M NH4OH), forming a clear, colorless solution.
What is cordycepin level in Cordyceps militaris?
The conventional extraction methods of cordycepin from C. militaris fruiting bodies are water based or alcohol based. However ultrasonic-assisted extraction (5) or enzyme-assisted extraction (6) have also been attempted yielding cordycepin levels as high as 86.98% and 86.45%, respectively.
What is the mechanism of action of cordycepin?
Wonga et al. (2011) Open in a separate window Cordycepin: mechanism of action The structure of Cordycepin is very much similar with cellular nucleoside, adenosine (Fig. 1) and acts like a nucleoside analogue. Inhibition of purine biosynthesis pathway
Is cordycepin a bioactive metabolite with therapeutic potential?
Tuli H.S., Sharma A.K., Sandhu S.S., Kashyap D. Cordycepin: A bioactive metabolite with therapeutic potential. Life Sci. 2013;93:863–869. doi: 10.1016/j.lfs.2013.09.030.
Does cordycepin work on cancer cells?
Thus, the results suggested that cordycepin works on cancer cells through the ADORA-G-protein-PLC pathway to induce cell apoptosis [11]. Phospholipase C (PLC) is significant in transmembrane signaling [63].
How does cordycepin inhibit UVB-induced matrix metalloproteinase expression?
Cordycepin inhibits UVB-induced matrix metalloproteinase expression by suppressing the NF-κB pathway in human dermal fibroblasts. Exp Mol Med. 2009;41:548–554.
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